Divergent regeneration-competent cells adopt a common mechanism for callus initiation in angiosperms. Molecular mechanisms of plant regeneration. An efficient and reproducible Agrobacterium-mediated transformation method for hexaploid wheat ( Triticum aestivum L.). De novo shoot organogenesis during plant regeneration. Shoot regeneration: a journey from acquisition of competence to completion. Cellular, molecular, and physiological aspects of in vitro plant regeneration. in Plant Tissue Culture: 100 Years Since Gottlieb Haberlandt (eds Laimer, M. Experimental validations highlighted TaDOF5.6 ( TraesCS6A02G274000) and TaDOF3.4 ( TraesCS2B02G592600) as potential enhancers of transformation efficiency in different wheat varieties. Further comparisons between wheat and Arabidopsis revealed distinct patterns of DNA binding with one finger (DOF) TFs in the two species. The built-up TRN driving wheat regeneration was found to be dominated by 446 key transcription factors (TFs). Our results demonstrate that the sequential expression of genes mediating cell fate transition during regeneration is induced by auxin, in coordination with changes in chromatin accessibility, H3K27me3 and H3K4me3 status. ![]() RNA-seq, ATAC-seq and CUT&Tag techniques were utilized to profile the transcriptional and chromatin dynamics during early regeneration from the scutellum of immature embryos in the wheat variety Fielder. ![]() Here we employed a multi-omic analysis strategy to uncover the transcriptional regulatory network (TRN) responsible for wheat regeneration. Genetic transformation is important for gene functional study and crop improvement.
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